The cell proliferation, cell cycle and apoptosis effects of cycloartane triterpenoid on HCT116 cells
10.3969/j.issn.1001-1978.2018.01.020
- VernacularTitle:环阿尔廷烷型四环三萜化合物对HCT116细胞增殖、细胞周期和凋亡的影响
- Author:
li Xiao DAI
1
;
Jing LIU
;
Yin NIAN
;
hua Ming QIU
;
hong Ji ZHANG
Author Information
1. 昆明理工大学医学院衰老与分子遗传学实验室
- Keywords:
Cimicifuga;
Cycloartane triterpeneoid;
colorectal cancer;
proliferation inhibition;
apoptosis;
periodic block;
anti-tumor
- From:
Chinese Pharmacological Bulletin
2018;34(1):91-96
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the antitumor effects of cimigenol ( KY17 ) , a novel cycloartane triterpenoid from Cimicifuga , in human colon cancer cells (HCT116).Methods MTT assay was used to deter-mine the effect of KY17 on the proliferation of mouse embryonic fibroblasts ( MEF) and human colon cancer HCT116 cell line.Flow cytometry was employed to de-tect the effect of KY17 on HCT116 cell cycle.Fluores-cence microscopy and flow cytometry were used to ana-lyze the apoptosis .Western blot was used to detect the expression of apoptotic protein (PARP).q-PCR ana-lyzed the expression of miRNA-34a.Results The IC50 of KY17 in MEF and HCT116 cells was 27.28 μmol· L-1 and 9.31μmol· L-1 , respectively.KY17 induced HCT116 cell cycle arrest in G2/M phase and the apoptotic protein PARP cleavage . In addition, KY17 up-regulated the expression of p 53 protein and miRNA-34a.Conclusions KY17 inhibits the prolifera-tion and the cell cycle is arrested in G 2/M, inducing the apoptosis of HCT116 cells. The mechanism is probably related to miRNA-34a up-regulation and p53 activation .
