Protective effect of cornel iridoid glycoside on hepatocytes injured by D-galactosamine/tumor necrosis factor-α
10.3969/j.issn.1001-1978.2018.01.025
- VernacularTitle:山茱萸环烯醚萜苷对D-GalN/TNF-α损伤肝细胞的保护作用研究
- Author:
xia Yan MA
1
;
hua Mian WU
;
qun Ze JIANG
;
ming Feng ZHAO
;
Li LI
;
han Mu LI
;
lin Tu LU
;
wu Ai HANG
Author Information
1. 南京中医药大学 基础医学院中药学教研室
- Keywords:
cornel iri doid glycoside;
D-galac-tosamine;
tumor necrosis factor-α;
liver damage;
anti-oxidation;
endoplasmic reticulum stress;
live protection
- From:
Chinese Pharmacological Bulletin
2018;34(1):118-122
- CountryChina
- Language:Chinese
-
Abstract:
Aim To determine the effect of cornel iri-doid glycoside ( CIG ) on human hepatocyte cell line (L-02) injured by D-galactosamine (GalN) and tumor necrosis factor-α( TNF-α) .Methods Firstly, CIG was extracted , separated and purified . Cell lesion model injured by D-GalN/TNF-αwas tested by MTT method.T-AOC, SOD, MDA and calcium ion concen-tration were taken as indicators to study the effects of CIG on L-02 cell injured by D-GalN/TNF-α.The ex-pression of p-PERK, p-eIF-2α, caspase-3 protein were detected by Western blot .Results 44 mg · L-1 D-GalN and 100 μg · L-1 TNF-αwere suitable for L-02 cell lesion model.CIG high, middle, low concentra-tion group could significantly increase the L-02 cell ac-tivity by 21％, 13％, 8％, respectively and SOD activity and T-AOC ability as well compared with model group.At the same time, they markedly reduced the MDA activity except the low concentration .Three con-centrations of CIG could reduce the expression of endo-plasmic reticulum stress related protein PERK , eIF-2αand apoptosis-associated protein caspase-3. Conclu-sions CIG could protect L-02 cells injured by D-GalN/TNF-α.Increasing the cellular antioxidant abili-ty, reducing the damage of endoplasmic reticulum stress and the expression of apoptosis-associated protein may be the possible mechanism .
