Protosappanin A increases the sensitivity of gastric cancer SGC-7901 cells to radiotherapy
10.11904/j.issn.1002-3070.2017.06.004
- VernacularTitle:原苏木素A对胃癌SGC-7901细胞系放疗增敏作用的研究
- Author:
Guohui LIU
1
;
Anxin GU
;
Hongtao YIN
;
Yang CAO
;
Yunlong HE
;
Chunbo WANG
;
Mingyan E
Author Information
1. 哈尔滨医科大学附属肿瘤医院胸部放疗二病区 哈尔滨 150081
- Keywords:
Protosappanin A;
Caesalpinia Sappan L extract;
Gastric cancer;
Radiotherapy;
Radiosensitiv-ity
- From:
Practical Oncology Journal
2017;31(6):500-505
- CountryChina
- Language:Chinese
-
Abstract:
Objective In this study,Protosappanin A,Caesalpinia Sappan L extract and Cisplatin were combined with radiotherapy in gastric cancer cell SGC-7901 to investigate whether the Protosappanin A could in-crease radiosensitivity( SER) in gastric cancer SGC-7901 cells. This will be medication to create new areas of in-novation in the future. Methods The cell proliferation of SGC-7901 cells was detected by MTT assay. The rela-tionship between the effect of the Protosappanin A on cell proliferation and the time of action was determined. Caesalpinia Sappan L extract and Cisplatin were as controls. The fitted cell survival curve and clonal formation as-says were used to determine the SER to analyze the sensitizative effect of Protosappanin A. Results Protosappa-nin A could inhibit the growth of SGC-7901 cells,and its inhibitory effect is relatively weak. Its cytotoxicity has a time-and concentration-dependent manner. Cellular morphological changes were observed accompanying with increased concentrations and time treatments of Protosappanin A. Clonal formation experiment showed that the Protosappanin A significantly increased the radiosensitivity of SGC-7901 cells when compared to the radioactive group. They showed a statistically difference. Conclusion The inhibitory effect of the Protosappanin A on SGC-7901 cells in a concentration and time-dependent manner. Protosappanin A combined radiotherapy can improve the radiosensitization of cells,both of which may have synergistic anti-tumor effect.
