Application of three-line positioning method in blood collection by cardiac puncture from rats

VernacularTitle:三线定位法在大鼠心脏采血中的应用
Author: tong Ling ZHOU ¹ ; Min SONG ; yu Xiao LIU ; bo Lin JIANG ; Kai HUANG ; tao Wan DONG ; rong Wan KANG
Author Information

1. 甘肃中医药大学
Keywords: three-line positioning method; blood collection from the heart; improved method; rat
From: Journal of Regional Anatomy and Operative Surgery 2017;26(12):919-921
CountryChina
Language:Chinese
Abstract: Objective To introduce an improved method for collecting the blood of rat by cardiac puncture .Methods The study se-lected 90 Wistar rats,half male and half female ,and then the rats were anesthetized by intraperitoneally injecting 10％chloral hydrate solution according to 0.3 mL/kg body weight.Then using three-line positioning method to determine the puncture site ,the blood collection needle and blood collection tube were used to collecting the blood ,Finally,the blood volume of each rat was recorded .Results The success rate was cal-culated according to the single blood collection of 5 mL or more,and our 90 rats were collected 90 times,the success rate was about 82.2％. After centrifugation,the total serum was about 260 mL,the average serum separation of each rat was about 2.8 mL.Conclusion The im-proved positioning method of heart blood collection is intuitive and easy to learn ,and the total volume and quality of blood is high ,besides ,the new method has a small surgical trauma and a high efficiency ,which is worth to extend .