Salvianolic acid B ameliorates cardiac hypertrophy in T2 DM mice by activating PPARα
10.3969/j.issn.1001-1978.2018.01.021
- VernacularTitle:丹酚酸B通过PPARα抑制2型糖尿病小鼠心肌肥厚
- Author:
mei Hui ZANG
1
;
Lei SU
;
zheng Jia CHEN
;
Tao YAN
;
mao Jia LIN
;
jun Li FANG
Author Information
1. 山东大学临床医学院
- Keywords:
salvianolic acid B;
type 2 diabetes;
cardi-ac hypertrophy;
high glucose;
high insulin;
peroxi-some proliferator activated receptor-α.
- From:
Chinese Pharmacological Bulletin
2018;34(1):97-102
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective role of salvianolic acid B ( Sal B ) on cardiac hypertrophy in type 2 diabetes mice , and to explore its influence on peroxisome proliferator activated receptors-α( PPARα) .Methods The type 2 diabetes melitus ( T2DM) mouse model was established by 4 weeks ' high fat diets feeding and one time STZ intraperitoneal injection .The animals were randomly divided into:control, T2DM, T2DM+SalB(100 mg· kg -1 · d-1 ) and Sal B(100 mg· kg -1 · d-1 ) groups.Eight weeks later, heart weight, tibial length, cross section area of cardiomyocytes , protein expression of PPARαin heart tissue were recorded .In vitro, high glucose and high insulin ( HGI ) were used to induce hypertrophic growth in cultured neonatal rat cardiomyocytes ( NRC-Ms) .And cell surface area , 3 H-leucine incorporation , 3 H-D-glucose uptake and PPARαprotein level were measured to observe the effect of Sal B and MK 886, a PPARαinhibitor.Results In T2DM model mice, Sal B could lower heart weight/tibial length and cross sec-tion area of cardiomyocytes , while PPARαprotein level in hearts were improved .In cultured cardiomyocytes , Sal B ( 10 ～100 μmol · L-1 ) ameliorated the in-creased levels of cell surface area ,3 H-leucine incorpo-ration and improved the decreased 3 H-D-glucose up-take and PPARαexpression induced by HGI . But those function could be abolished by MK 886.Conclu-sion Sal B ameliorates cardiac hypertrophy in T 2DM mice, which may be related to its function on PPARαactivation .
