Specific sIgA antibodies against swine influenza virus H3N2 infection
10.3969/j.issn.1002-2694.2017.11.002
- VernacularTitle:猪流感H3N2型病毒特异性sIgA抗体分泌规律研究
- Author:
Yun BAI
1
;
song Cai SHU
;
Yuan GAN
;
Bo NI
;
zhong Li HUA
;
Meng WU
;
qing Guo SHAO
;
xin Zhi FENG
Author Information
1. 江苏省农业科学院兽医研究所农业部兽用生物制品工程技术重点实验室
- Keywords:
swine influenza virus;
indirect ELISA;
sIgA antibodies
- From:
Chinese Journal of Zoonoses
2017;33(11):956-961
- CountryChina
- Language:Chinese
-
Abstract:
To study different breed pigs reply the swine flu virus infections,specific antibody of sIgA secretion regularity of respiratory tract and the differences of sIgA antibody according to different antigen proteins were detected.A/swine/Nanjing/ 51/2010(H3N2) was intranasally infected pigs (1 × 107 TCID50/mL and 2 mL/pig),and then the nasal swab samples were collected at different time points within 21 days after infection.M1,NS1 and PB1 recombinant protein,respectively,were used to establish indirect ELISA method for detecting specific antibody of sIgA,and to analyze its secretion regularity.Results displayed that there was no significant difference among three kinds of recombinant protein in the whole test,characterizing by specificity sIgA antibody levels rising rapidly after 5 infection days and reaching peak at day 14,then began to decline.Among different varieties of pigs,sIgA antibody production of PB1 protein in Obama group was significantly higher than that in binary pigs at 14th and 21st day (P＜0.05).It had no significant difference between M1 group and the NS1 group (P＞0.05).This experiment preliminary explores the secretion regularity of specificity sIgA antibody after infected swine flu virus,which laid a foundation for further study of SIV mucosal antibody diagnostic reagents.
