Distribution characterization of 29 virulence genes in 91 foodborne Listeria monocytogenes strains
10.3969/j.issn.1002-2694.2017.11.005
- VernacularTitle:29种毒力基因在91株食源性单核细胞增生李斯特氏菌中的分布
- Author:
juan Xiu LI
1
;
Dong ZHAO
;
Zhuo PAN
;
li Wei GAO
;
hong Bao XU
;
yun Li QIN
Author Information
1. 石家庄市疾病预防控制中心
- Keywords:
Listeria monocytogenes;
virulence genes;
distribution characteristics
- From:
Chinese Journal of Zoonoses
2017;33(11):972-978
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the distribution of virulence genes of Listeria monocytogenes (Lm) in Hebei Province,29 virulence genes of Lm,including Listeria monocytogenes pathogenicity islands Ⅰ (LIPI-Ⅰ:prfA,plcA,plcB,hlyA,mpl and actA),10 internalins genes (inlA,inlB,inlC,inlD,inlE,inlF,inlG,inlH /C2,inlI and inlJ) and the other 13 virulence-associated genes (bsh,srtA,iap,sigB,virR,mprF,dltA,dltB,dltC,dltD,srtB,fbpA and hpt) were detected by PCR.Results showed that in the 91 Lm strains,the detection rate of 23 virulence genes were 100%.The 29 virulence genes of 26 Lm strains were all detected,and 65 Lm strains had different deletion of 6 virulence genes inlD,inlF,inlG,inlH /C2,inlJ and mpl.The deletion rate of inlG and inlF were 60.44% and 54.95%,respectively,following by mpl gene,with a deletion rate of 19.78%.According to the absence of virulence genes,91 strains could be divided into 10 subtypes,and the dominant virulence subtypes was type Ⅰ with all 23 virulence genes.The deletion rate of virulent genes in Shijiazhuang was higher than that in northern Hebei.It is suggested that the rate of virulence gene of food-borne Lm in Hebei Province is high,and the virulence gene deletion patterns has diversity and regional differences.