Proportion of regulatory T cells and expression of interleukin-17A and-27 in the peripheral blood of patients with chronic spontaneous urticaria and their significance
10.3760/cma.j.issn.0412-4030.2017.12.014
- VernacularTitle:慢性自发性荨麻疹患者外周血Treg细胞比例和白细胞介素17A、27水平及意义
- Author:
Yating WANG
1
;
Chunrui SHI
;
Yi WU
;
Bei SHI
;
Xiao XIONG
;
Lanxia ZHOU
Author Information
1. 730000,兰州大学第一临床医学院兰州大学第一医院皮肤性病科
- Keywords:
Urticaria;
Interleukin-17;
T-lymphocytes,regulatory;
Interleukin-27
- From:
Chinese Journal of Dermatology
2017;50(12):916-919
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of peripheral blood CD4+CD25+CD127low Treg cells,interleukin-17A (IL-17A) and IL-27 in the pathogenesis of chronic spontaneous urticaria (CSU).Methods Peripheral blood samples were obtained from 37 patients with CSU (CSU group)and 40 healthy controls (control group).Flow cytometry was performed to determine the percentage of CD4+CD25+CD127low Treg cells in the peripheral blood,and enzyme-linked immunosorbent assay (ELISA) to detect the serum levels of IL-17A and IL-27.Results The percentage of CD4+CD25+CD127low Treg cells in the peripheral blood (5.99％ ± 2.72％ vs.9.07％ ± 3.44 ％,t =4.325,P ＜ 0.01) and the serum level of IL-27 (20.54 ± 7.65 ng/L vs.26.63 ± 9.72 ng/L,t =3.039,P =0.003) were both significantly lower in the CSU group than in the control group.However,there was no significant difference in the serum level of IL-17A between the 2 groups (P =0.529).Among the patients with CSU,the level of IL-17A was negatively correlated with the percentage of CD4+CD25+CD127low Treg cells (r =-0.359,P =0.029),while the urticaria activity score (UAS) was uncorrelated with the levels of IL-17A and IL-27 as well as the percentage of CD4+CD25+ CD127low Treg cells (r =-0.076,-0.083,-0.053 respectively,all P ＞ 0.05).Conclusion There may be Th17/Treg imbalance in the peripheral blood of patients with CSU,and IL-27 may be involved in the occurrence of CSU.
