Quercetin Inhibited ADMA-induced Endoplasmic Reticulum Stress Responses and HUVECs Apotosis
10.11969/j.issn.1673-548X.2017.11.010
- VernacularTitle:槲皮素抑制ADMA引起的脐静脉内皮细胞内质网应激和细胞凋亡
- Author:
Weikang GUO
1
;
Yingjie LIU
;
Wenhu LIU
Author Information
1. 100050,首都医科大学附属北京友谊医院肾内科
- Keywords:
ADMA;
ER stress;
Apoptosis;
Quercetin
- From:
Journal of Medical Research
2017;46(11):34-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of quercetin on ADMA induced endoplasmic reticulum stress responses (ER stress).Methods Human umbilical veins endothelial cells (HUVECs) were cultured,and left untreated or challenged for 24h with 100μmol/L ADMA in the absence and presence of 20μmol/L quercetin,the PERK and IRE1 was determined by Western blot.ATF4 and CHOP mRNA was detected by RT-PCR.Cells apoptosis was detected by flow cytometry.Results ADMA induced HUVECs apoptosis.Quercetin inhibited ADMA-induced PERK and IRE1 protein expression and ATF4 and CHOP mRNA expression.At last,Quercetin inhibited ADMA-induced cells apoptosis.Conclusion ADMA induced ER stress and HUVECs apotosis while quercetin inhibited ADMA-induced ER stress and apoptosis.
