Effects of low temperature compound propofol on expression and ultrastructural changes of hippocampal neurons apoptosis proteinin rats
10.3969/j.issn.1671-8348.2017.34.006
- VernacularTitle:低温复合丙泊酚对大鼠海马神经元凋亡蛋白表达及超微结构变化的影响
- Author:
Ya HE
1
;
Xiaoying LIU
;
Qinghua DENG
;
Xiaohong ZHENG
;
Hongyan JIANG
;
Yuanqi SU
;
Lan PENG
Author Information
1. 重庆市渝北区人民医院麻醉科 401120
- Keywords:
hypothermia;
propofol;
hippocampal;
neurons;
apoptosis
- From:
Chongqing Medicine
2017;46(34):4774-4776
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of low temperature compound propofol on the changes of apoptosis protein Caspase-3,autophagy-related proteins Beclin-1 and LC3-Ⅱ and thier changes of hippocampal neurons.Methods The rats were randomly divided into blank control group (Group A),propofol group at low temperature (Group B) and chloral hydrate group at low temperature (Group C),Group B and C were treated with low temperature for 30 min.Then,each group was subjected to cardiac perfusion and decapitated brain to prepare rat hippocampal neuronal tissue samples.The expression of Caspase-3 was detected by immunohistochemistry,Beclin-1 and LC3-Ⅱ protein was detected by immunoblotting.The ultrastructural changes of neurons were observed by transmission electron microscopy.Results The expression of Caspase-3,Beclin-1 and LC3-Ⅱ protein in each group were statistically significant differences(P＜0.05).The results of transmission electron microscopy showed that the neurons in group B and C were changed in different degrees,especially in group C neuronal apoptosis is obvious.Conclusion Autophagy and apoptosis in existence still exist in low temperature condition,while propofol can reduce this damage and have better protective effect on neurons.
