Pathologic staining method improvement for detection of pulmonary cryptococcal disease
10.3760/cma.j.issn.1006-9801.2017.12.010
- VernacularTitle:肺部隐球菌病检测的病理染色方法改进
- Author:
Xiuqin WENG
1
;
Shijuan YANG
;
Zhisheng XIANG
;
Gang CHEN
Author Information
1. 福建省肿瘤医院病理科
- Keywords:
Lung diseases;
Cryptococcosis;
Pathology,clinical;
Staining and labeling;
Silver staining;
Hexamine silver
- From:
Cancer Research and Clinic
2017;29(12):841-843
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the pathological staining method of pulmonary cryptococcal disease. Methods Traditional hexamine silver staining and acidified potassium permanganate solution hexamine silver stained were used to analyze paraffin sections of 51 patients with pulmonary cryptococcal disease. Results Compared with traditional hexamine silver stained, the result of acidified potassium permanganate solution with hexamine silver stained was positive, and the whole process only required 25 minutes with the clear background and cryptococcal cyst capsule structure. Conclusion Compared with the traditional hexamine silver staining, acidification of potassium permanganate solution hexamine silver staining method is simpler,easier,more effective and worthy of wide application.