125 cases of children with epilepsy peripheral blood B lymphocytes ,NK cells and NKT cells in the detection and analysis
10.3969/j.issn.1673-4130.2017.23.024
- VernacularTitle:125例癫痫患儿外周血B淋巴细胞、NK细胞和NKT细胞的检测与分析
- Author:
Yonghong HUANG
1
;
Ying KANG
;
Dengke HAN
;
Qiong CHEN
;
Shengnan XU
Author Information
1. 中山市港口医院内儿科
- Keywords:
epilepsy;
CD19;
CD20;
NK cells;
NKT cells
- From:
International Journal of Laboratory Medicine
2017;38(23):3286-3287,3290
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes and clinical significance of CD19 ,CD20 ,CD3-CD16+ CD56+ (NK cells) and CD3+CD16+CD56+ (NKT cells) in the children with epilepsy .Methods 125 cases of children with epilepsy children confirmed by Pediatric neurology specialists in Zhongshan People's Hospital from January 2013 to March 2016 were selected as epilepsy group , while 125 cases of healthy children were selected as control group ,and the levels of CD19 ,CD20 ,NK cells and NKT cells in periph-eral blood of two groups were detected by flow cytometry .Results CD19 ,CD20 ,NK cells and NKT cells levels in epilepsy group were higher than those in control group ,and the difference was statistically significant (P<0 .05) .For ROC curve analysis ,CD19 optimal cutoff value of 9 .4 ,the area under the curve was 90 .3% ,with 91 .7% sensitivity .CD20 optimal cutoff value of 7 .75 ,the ar-ea under the curve was 87 .3% ,with 91 .7% sensitivity .NK cells optimal cutoff value of 8 .55 ,the area under the curve was 80 .5% , with 95 .8% sensitivity .NKT cells optimal cutoff value of 0 .35 ,the area under the curve was 76 .9% ,with 91 .7% sensitivity .Con-clusion CD19 ,CD20 ,NK cells and NKT cells can be used as important indexes for monitoring immune conditions in children with epilepsy .
