Comparison on the preparation of rat models of pneumonia by different infection route
10.3969/j.issn.1005-4847.2017.06.003
- VernacularTitle:不同感染途径致大鼠肺炎模型制备的比较
- Author:
ying Jun DING
1
;
Xiang GAO
;
ying Yan HONG
;
Hong GUI
;
you Zhi WEI
;
fei Xue DING
;
ran You LU
;
dong Shi AN
;
ran Xu CUI
;
Qing-quan YU-HONG
;
LIU GUO
Author Information
1. 首都医科大学附属北京中医医院 北京市中医研究所 中医感染性疾病基础研究北京市重点实验室
- Keywords:
Pseudomonas aeruginosa;
Drug resistant bacteria;
Pneumonia;
Inflammatory reaction;
Rats
- From:
Acta Laboratorium Animalis Scientia Sinica
2017;25(6):600-604
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the preparation of a rat model of pneumonia model induced by Pseudomonas aeruginosa( PA) using different methods,and to lay the foundation for further studies. Methods 48 SD rats were randomly divided into 4 groups:the control group (A), the intratracheal injection group (B), the trachea cannulation group (C) and the intranasal inoculation group ( D) . After intervention with different treatment modalities, the body weight,tempera-ture,white blood cell count and lung pathological changes in the rats of all groups were detected at 5, 10, 15 days. Results 1. The behavior, body weight, temperature, leukocytes and pathological inflammatory changes of the lung in rats of the model groups were significantly different from that of control group. 2. Pseudomonas aeruginosa was detected in rats of all the model groups, but the control group was negative. Conclusions Rat model of Pseudomonas aeruginosa infected pneu?monia can be successfully established by intranasal inoculation. This method can avoid the inflammatory interference from operation, and is simple and suitable for popularization.
