Establishment of a modified in vitro mongolian gerbil oocyte fertilization and embryo culture system
10.3969/j.issn.1005-4847.2017.06.007
- VernacularTitle:长爪沙鼠体外受精与早期胚胎体外培养体系的初步建立
- Author:
gang Hong GUO
1
;
Li LI
;
lai Sheng ZHOU
;
qun Ling LU
;
Kun LI
;
tao Jiang DU
;
juan Qiao SHI
;
qing Xiu JIN
;
long Chang LI
Author Information
1. 浙江省医学科学院
- Keywords:
Mongolian gerbil;
In vitro fertilization;
In vitro culture
- From:
Acta Laboratorium Animalis Scientia Sinica
2017;25(6):624-631
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of self-made capacitation liquid for in vitro fertilization of Mongolian gerbils, and to provide a reference for gerbil embryo cryopreservation. Methods In vitro fertilization of Mongo?lian gerbil was performed with the self?prepared capacitation solution and semen, and the 2?cell embryos of Mongolian ger?bils were cultured in vitro using an improved KSOM culture medium. Results The in vitro fertilization rate of gerbils was over 60%, and some gerbil 2?cell embryos could develop further in vitro. Conclusions An in vitro fertilization and embry?o development system of Mongolian gerbil has been established, but it needs further optimization.
