Effects of Atractylenolide Ⅰ,Atractylenolide Ⅱ and Atractylenolide Ⅲ on Cytokines Expression of Inflam-matory Macrophages
- VernacularTitle:白术内酯Ⅰ、Ⅱ、Ⅲ对炎性巨噬细胞细胞因子表达的影响
- Author:
Qinhua CHEN
1
;
Fei YU
;
Hongmei WANG
;
Xueru DING
;
Jun ZHU
;
Yushuang LIU
;
Lun ZHANG
Author Information
1. 湖北医药学院附属东风医院 湖北十堰442008
- Keywords:
Atractylenolide;
Inflammatory macrophage;
Anti-inflammatory
- From:
China Pharmacist
2017;20(12):2112-2116
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of atractylodes lactideⅠ,Ⅱand Ⅲ on the expression of cytokines by inducing M1 type macrophage model. Methods:Apoptotic macrophages were induced by sodium thioglycolate, and then the rat peritoneal inflammatory macrophages were purified by a differential adherence method. The expression of macrophage marker antigen (ED1) was identified by flow cytometry macrophages. Tumor necrosis factor ( TNF-α) , proinflammatory cytokines ( IL-1β) and interleukin-6 ( IL-6 ) were measured by ELISA in vitro. The levels of expression of arginase 1, inducible nitric oxide synthase (iNOS), inflammatory cytokines (IL-1β) chemokine (CCL22) and transforming growth factor (TGF-β) in inflammatory macrophages were determined by RT-PCR. Results:As for the purification of cultured rat inflammatory macrophage expressing ED1, atractylenolideⅠand Ⅲ could reduce the ex-pression levels of iNOS and IL-1βand increase the expression levels of arginase1 CCL22 and TGF-β. The release of inflammatory fac-tor IL-1β decreased, and the release levels of TGF-β and chemokines CCL22 were promoted(P<0. 05). Atropine lactoneⅠ and Ⅲ had significant inhibitory effects on TNF-α, IL-1β and IL-6 in macrophages(P<0. 05). Conclusion: Atractylodes lactone Ⅰand Ⅲ with anti-inflammatory activity can promote the expression of cytokines in inflammatory macrophages, while the change induced by at-ractylaxanthin Ⅱ is not obvious.
