In vitro Antitumor Activity of Iridoid Glycosides from Paederiascandens
- VernacularTitle:鸡矢藤环烯醚萜苷体外抗肿瘤活性研究
- Author:
Hongxia LI
1
;
Lei YANG
;
Xiaoli CHEN
;
Xiaolan DAI
;
Li XU
Author Information
1. 解放军第113医院药械科 浙江宁波315040
- Keywords:
Iridoid glycosides from paederiascandens;
Antitumor activity;
In vitro
- From:
China Pharmacist
2017;20(12):2117-2122
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the chemical compositions and anti-tumor activity of iridoid glycosides from Paederiascandens ( IGPS) . Methods:IGPS was prepared by ultrasonic extraction and macroporous resin separation and purification methods, and ana-lyzed the chemical compositions by LC-MS. MTT was used to study the proliferation inhibition effects of IGPS on the proliferation of hu-man fibroblast cell line NIH-3T3 and human lung cancer cell line A549 and SPC-A-1, human colon cancer cell line COLO205 and HCT-116, human leukemia cell line HL-60 and K562, human breast cancer cell line MCF7 and BT-549, human gastric adenoma cell line SGC7901 and human cervical cancer cell line Hela. Flow cytometry was used to analyze the cell apoptosis of SGC7901, Hela and HCT-116 cell line after treated with IGPS. Results:The extraction and purification of IGPS obtained paederoside, scandoside, paed-erosidic acid and asperuloside. Compared with the negative control group, IGPS at the concentration less than or equal to 500 μg· ml-1 were non-toxic to the normal cell NIH-3T3. IGPS could inhibit the proliferation of Hela, HCT-116, COLO205, BT-549 and MCF7 cells in a dose-dependent manner (P<0. 05), while showed no inhibitory effect on lung cancer cell lines A549 and SPC-A-1, and human leukemia cell lines HL-60 and K562. IGPS had the strongest inhibitory effect on SGC7901 with the IC50 of 156. 6μg· ml-1 , and IGPS could induce the apoptosis of SGC7901, Hela and HCT-116 cells effectively. Conclusion:IGPS can inhibit the prolif-erations of many cancer cell lines.
