Optimization of Extraction Process for Total Lignans from Fructus Chinensis by Orthogonal Test
- VernacularTitle:正交试验法优化五味子总木脂素的提取工艺
- Author:
Yingli LI
1
;
Zhijun ZHU
;
Nannan ZHANG
;
Hao DENG
;
Song GAO
;
Ying LI
Author Information
1. 河南中医药大学药学院 郑州450008
- Keywords:
Total lignans from Schisandra chinensis;
Orthogonal experimental method;
Alcohol extraction
- From:
China Pharmacist
2017;20(12):2139-2142
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the extraction process of the total lignans from Fructus chinensis. Methods:The comprehensive weighted score method for the extraction rate and the extraction content was regarded as the index by orthogonal experimental method, the effects of ethanol concentration, extraction duration, amount of ethanol and extraction times on the extraction of the total lignans from Fructus chinensis were studied. The chromatographic separation was performed on a Unitary C18 column (250 mm × 4. 6 mm,5μm). Methanol(A)-water(B) was used as the mobile phase (0-10 min:70% A;10-13 min:70% A-85% A;13-30 min:85% A). The detection wavelength was set at 217 nm, the column temperature was maintained at 30℃,the flow rate was 1. 0 ml·min-1 and the sample size was 10μl. Results:The extraction conditions of the total lignans were as follows:14-fold amount of 85% ethanol was used for 3 times extraction with 2. 5 h for each time. Under the above conditions, the extraction rate of Schisandra lignans was 1. 10%, and the extraction content was 22. 99%. Conclusion:The extraction and purification technology for Schisandra lignans and the total poly-saccharides is stable and reliable, which provides scientific basis for the reform of Shengmai new dosage forms.
