Effect of Enriched Environment on Neuroregeneration in Subventricle Zone of Rats with Vascular Dementia
10.3969/j.issn.1006-9771.2017.12.004
- VernacularTitle:丰富环境对血管性痴呆大鼠侧脑室室管膜下区神经再生的影响
- Author:
Xu HE
1
;
fei Ying LIU
;
ying Ming LUO
;
wu Shao CHENG
;
wen Jin GE
Author Information
1. 益阳医学高等专科学校解剖教研室
- Keywords:
vascular dementia;
enriched environment;
subventricle zone;
neuroregeneration;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2017;23(12):1384-1389
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of enriched environment on neuroregeneration in subventricle zone(SVZ)of rats with vascu-lar dementia.Methods All rats were divided into sham group(n=5),vascular dementia group(VD group,n=12)and enriched environment group(EE group,n=12).Then,2-VO method was applied to make the vascular dementia model.The sham group and VD group received conventional breeding environment for 30 days,while EE group was subjected to enriched environment for 30 days.Immunohistochemistry was applied to detect the BrdU and Doublecortin(DCX)expression in SVZ,and DCX/Ki67,DCX/p-cAMP-response element binding pro-tein(CREB)expression in SVZ was assessed by immunofluorescence double-labeling technique.Results Compared with the sham group, the number of DCX+cells(t=2.989,P=0.026)and BrdU+cells(t=3.069,P=0.005)increased in VD group,and increased more in EE group (t=3.067, P=0.027; t=2.907, P=0.011). Besides, the number of the DCX/Ki67 (t=2.994, P=0.040) and DCX/p-CREB (t=4.707, P=0.009) cells was significantly higher in EE group than in VD group.Conclusion Enriched environment could up-regulate the neuroregeneration ca-pacity in SVZ of rats with vascular dementia through CREB signal pathway.
