Determination of Ginsenoside Rg1 ,Ginsenoside Re and Ginsenoside Rb1 in Xueluotong Capsules by HPLC
- VernacularTitle:HPLC法测定血络通胶囊中人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量
- Author:
Xiaoyan HUANG
1
;
Shi LUO
;
Ruilian LI
;
Zhenyu PAN
;
Jigang ZHANG
Author Information
1. 湖南省药品检验研究院 长沙410001
- Keywords:
Xueluotong capsules;
Ginsenoside Rg1;
Ginsenoside Re;
Ginsenoside Rb1;
HPLC;
Content determination
- From:
China Pharmacist
2017;20(12):2248-2250
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of ginsenoside Rg1 ,ginsenoside Re and ginsenoside Rb1 in Xueluotong capsules. Methods:A column of Waters Symmetry C18 ( 250 mm × 4. 6 mm,5 μm) at the temperature of 35 ℃ was used to separate the target components, the mobile phase consisted of acetonitrile-water with gradient elution, the flow rate was 1. 0 ml ·min-1 , the detection wavelength was 203 nm and the injection volume was 10 μl. Results:The linear range of ginsenoside Rg1 was 0. 055-2. 732 μg(r=0. 9998), and the average recovery was 107. 23% with RSD of 1. 17%(n=6). The linear range of ginsenoside Re was 0. 341-8. 542 μg(r=0. 9999), and the average recovery was 101. 63% with RSD of 3. 52%(n=6). The linear range of gin-senoside Rb1 was 0. 717-14. 336 μg(r=0. 9997), and the average recovery was 100. 63% with the RSD of 3. 79%(n=6). Conclu-sion:The method is simple, accurate and reliable, which can be used for the quality control of Xueluotong capsules.
