Quality Standard for Sancao Anshen Capsules
- VernacularTitle:三草安神胶囊的质量标准
- Author:
Fang CHEN
1
;
Yijie WANG
;
Xin LI
;
Guohua ZHENG
;
Junjie HU
Author Information
1. 湖北中医药大学 武汉430065
- Keywords:
Sancao Anshen capsules;
Quality standard;
Rosmarinic acid;
TLC;
HPLC
- From:
China Pharmacist
2017;20(12):2255-2257
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the quality standard for Sancao Anshen capsules. Methods:Valerian, Spica Prunellae, Radix Scutellariae and Rhizoma Coptidis were indentified by TLC. The content of rosmarinic acid was determined by HPLC. The column was Hypersil ODS2 C18 with mobile phase of acetonitrile-0. 1% trifluoroacetic acid(30:70,V/V) at a flow rate of 1. 0 ml·min-1 , the col-umn temperature was 25 ℃ ,the detection wavelength was 330 nm,and the sample size was 20 μl. Results: The characteristic spots could be detected by TLC with good reproducibility. The linear range of rosmarinic acid was 0.105-2.104 μg·ml-1(r=0.9993). The average recovery was 99. 5% and the RSD was 0. 80% (n=6). Conclusion:The developed method is simple and accurate, and suitable for the quality control of Sancao Anshen capsules.
