Development of Neutralization Assay using Murine Leukemia Virus (MuLV) Pseudotyped with Japanese encephalitis Virus (JEV) env Gene.
- Author:
Hee Jung LEE
1
;
Kyung Il MIN
;
Nuri PARK
;
Go eun BAE
;
Jae Hwan NAM
;
Sook Jin HUR
;
Young Bong KIM
Author Information
- Publication Type:Original Article
- Keywords: JEV; Envelope (E); Pseudotyped virus; Neutralization
- MeSH: Animals; Antibodies, Neutralizing; Asian Continental Ancestry Group*; Encephalitis Virus, Japanese*; Encephalitis, Japanese*; Genes, env*; Glycoproteins; Humans; Leukemia Virus, Murine*; Mice; Neutralization Tests; Vero Cells
- From:Journal of Bacteriology and Virology 2007;37(1):23-30
- CountryRepublic of Korea
- Language:Korean
- Abstract: The envelope (E) glycoprotein of JEV is the major antigen to elicit neutralizing antibody (NAb) against JEV infection. In order to develop a rapid and safe neutralization assay system for evaluation of the JEV vaccine strains, we constructed JEV-pseudotyped viruses with JEV env genes (Nakayama-NIH, Beijing-1). The titers of JEV-pseudotyped viruses with NK and BJ strains were 4.0x10(4) IFU/ml and 1.3x10(5) IFU/ml in Vero cell cultures, respectively. We have analyzed the neutralization activity of immunized mouse sera with JEV-NK and JEV-BJ pseudotyped viruses. The neutralizing antibody titers of NK and BJ (50% reduction of virus) were about 1:10,000 at each immunized sera. Compared with conventional plaque reduction neutralization test (PRNT), the method using JEV-pseudotyped virus has desirable advantages such as more rapid, easier, and non-biohazardous. This neutralization assay system might be useful to evaluate NAb activity against JEV vaccine strains or vaccine candidates.
