Study on the Th17 ,Th9 cells and the expression of the correlated cytokines in peripheral blood of patients with pediatric bronchial asthma and the clinical significance
10.3969/j.issn.1673-4130.2017.23.016
- VernacularTitle:支气管哮喘患儿外周血Th17和Th9细胞及其细胞因子的表达及临床意义研究
- Author:
Zhi LI
1
;
Tingting YANG
;
Wenzhe LI
;
Bo WANG
;
Banglu XUE
Author Information
1. 大连市中心医院检验科
- Keywords:
Pediatric bronchial asthma;
T helper 17;
T helper 9;
interleukin(IL)-17;
IL-9
- From:
International Journal of Laboratory Medicine
2017;38(23):3262-3264,3267
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the levels of helper T cells (Th) 17 and Th9 cells in the peripheral blood of patients with pedi-atric bronchial asthma and the expression of interleukin 17 (IL-17) ,IL-9 and total IgE in serum ,and to discuss their effect and clini-cal significance in pathogenesis of pediatric asthma .Methods A total of 46 children with bronchial asthma ,including asthma attack group (n=26) and asthma remission group (n=20) were selected .Healthy children were selected as healthy control group (n=20) .Flow cytometry was used to detect the percentages of Th17 and Th9 cells in the peripheral blood .The levels of IL-17 and IL-9 in serum were measured by enzyme-linked immunosorbent assay (ELISA) .Serum total IgE levels were measured by the specific protein analyzer .Results The percentages of Th17 and Th9 cells in the peripheral blood were significantly higher in asthmatic group compared with remission group and healthy control group (P<0 .05) .The percentages of Th17 and Th9 cells in the periph-eral blood was significantly higher in remission group compared with healthy control group (P<0 .05) .The levels of IL-17 ,IL-9 and total IgE in serum were significantly higher in asthmatic group compared with remission group and healthy control group (P<0 .05) .The levels of IL-17 ,IL-9 and total IgE in serum were significantly higher in remission group compared with healthy control group (P<0 .05) .The levels of IL-17 and IL-9 in serum of asthmatic children were positively correlated with total IgE levels (P<0 .05) .The correlation analysis results showed that the levels of IL-17 ,IL-9 and total IgE in serum of patients with pediatric bron-chial asthma have a positive correlation .(r=0 .717 ,0 .491 ,0 .786 ,P<0 .05) .Conclusion Th17 ,Th9 cells and their cytokines in pe-ripheral blood of patients with pediatric bronchial asthma play important roles in the pathogenesis of asthma and are positively cor-related with the severity of asthma .
