Ethanol promotes the progression of breast cancer in mice by activating endoplasmic reticulum stress
10.3969/j.issn.1005-4847.2017.06.004
- VernacularTitle:饮酒通过内质网应激促进小鼠乳腺癌恶性进展
- Author:
Pei ZHU
1
;
kun Ya LIU
;
Yu JIANG
;
ying Si WANG
Author Information
1. 安徽医科大学基础医学院病理生理学实验室
- Keywords:
Ethanol;
Breast cancer;
Endoplasmic reticulum stress;
Mice
- From:
Acta Laboratorium Animalis Scientia Sinica
2017;25(6):605-610,617
- CountryChina
- Language:Chinese
-
Abstract:
Objective Epidemiological studies have suggested that alcohol drinking is closely associated with di-verse tumor development, but fewer laboratory studies or mechanism analysis is made. Our study aims to explore the effect of alcohol on the tumorigenicity and metastasis of breast cancer in nude mice in vivo and on the malignant behavior in vitro, and whether it is related to endoplasmic reticulum stress ( ERS) . Methods We first established the nude mouse model of breast cancer with chronic alcohol consumption (2%) and to detect the effects of ethanol on tumor growth and metastasis. We also observed the changes of malignant biological behavior of EO771 cells in cell culture induced by chronic alcohol treatment (0. 2%) using MTT, Transwell and soft agar tests, and detect the expression of ROS and ERS marker, i. e. p?eIF2a, Bip, and XBP1?s. Results The in vivo experiment showed that alcohol drinking enhanced the growth and metasta?ses of breast cancer in nude mice. The in vitro test showed that chronic exposure to ethanol also promoted the cell prolifera?tion and migration ability, increased the ROS expression, activated ERS pathway, and enhanced the expression of p?eIF2a, Bip and XBP1?s. Conclusions Alcohol drinking may promote the malignant behavior of breast cancer cells through endo?plasmic reticulum stress.
