Optimization of Extraction Technology of Ilex Chinensis Sims by Box-Behnken Response Surface Method
- VernacularTitle:Box-Behnken响应面法优化四季青的提取工艺
- Author:
Hongnü GAN
1
;
Zongliang CHI
Author Information
1. 杭州市中医院 杭州310007
- Keywords:
Ilex chinensis Sims;
Pedunculoside;
HPLC;
Box-Behnken response surface method
- From:
China Pharmacist
2017;20(12):2149-2152
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the extraction process of Ilex chinensis Sims by Box-Behnken response surface method. Meth-ods:The HPLC determination of pedunculoside was performed and validated. With the extraction rate of pedunculoside as the index, the influencing factors including material-liquid ratio, ethanol concentration, reflowing temperature and decocting frequency were opti-mized by Box-Behnken response surface method. Results:The optimized parameters were as follows:the material/liquid ratio was 1:10, the ethanol concentration was 70%, the ultrasonic temperature was 80℃,and the decocting frequency was three times. Conclu-sion:The HPLC method and the best extraction conditions are reliable.
