Effect of Shuangteng Bitong Cataplasm on Inflammatory Cytokines in Plasma and Relevant Factors of Rats with Collagen-induced Arthritis
10.3969/j.issn.1005-5304.2018.01.010
- VernacularTitle:双藤痹痛凝胶膏剂对胶原诱导性关节炎模型大鼠血浆炎性因子和滑膜组织相关因子的影响
- Author:
hong Guo YAN
1
;
qin Yu ZHANG
;
Xiong LIN
;
Yan HUANG
;
qin Shu WENG
;
Wei XU
Author Information
1. 福建中医药大学附属人民医院
- Keywords:
Shuangteng Bitong Cataplasm;
collagen-induced arthritis;
TLR4;
MyD88;
NF-κB;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(1):43-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Shuangteng Bitong Cataplasm on TLR4, MyD88 and NF-κB and inflammatory cytokines in rats with collagen-induced arthritis; To discuss relevant mechanism of action. Methods A CIA model was made by subcutaneous injection of collagen emulsion from rat tail. Rats were randomly divided into normal group,model group,Shuangteng Bitong Cataplasm high-dose and low-dose groups.The contents of TNF-α, IL-1β and IL-6 in plasma were evaluated by ELISA, and the expression levels of TLR4, MyD88 and NF-κB in synovial tissues were evaluated by RT-PCR and Western blot. Results Compared with the model group, the contents of TNF-α, IL-1β and IL-6 in serum decreased remarkably in the Shuangteng Bitong Cataplasm low-dose and high-dose group, and the expression levels of TLR4, MyD88 and NF-κB in synovial tissues decreased remarkably. Conclusion Shuangteng Bitong Cataplasm can inhibit inflammatory reaction and regulate the expressions of relevant factors in synovial tissues to realize the anti-flammatory effects.