Effects of Different Dosages of Moxibustion with Ginger-Separated Moxibustion on MEK1/2 and ERK1/2 of Gastric Tissue in Rats with Spleen Deficiency
10.3969/j.issn.1005-5304.2018.01.012
- VernacularTitle:不同灸量隔姜灸对脾虚证大鼠胃组织丝裂原细胞外激酶和细胞外调节蛋白激酶表达的影响
- Author:
yan Ding BI
1
;
jie Jiong NING
;
Yin XU
;
Yan LUO
;
qing Mu LI
;
hui Yun WANG
;
Zhan YI
Author Information
1. 湖南中医药大学
- Keywords:
dosage of moxibustion;
ginger-separated moxibustion;
spleen deficiency;
proliferation and restoring of gastric mucosa;
mitogen activated protein kinase;
extracellular regulated protein kinase;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2018;25(1):54-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effects of different dosages of moxibustion with ginger-separated moxibustion on expressions of mitogen extracellular kinase (MEK) 1/2 and extracellular regulated protein kinase (ERK) 1/2 of gastric tissue in rats with spleen deficiency; To explore the possible mechanism and the dose-effect relationship. Methods Seventy-five SD rats were randomly divided into blank control group, model group, ginger-separated moxibustion for three zhuang group, six zhuang group and nine zhuang group according to random digits table method, with fifteen rats in each group. The rat model of spleen deficiency was established by intragastric administration with 200% Rhei Radix et Rhizoma infusion at 4 ℃. Ginger-separated moxibustion groups were treated with different dosage of moxibustion at "Zusanli", "Zhongwan" for eight days after the modeling. Pathological changes of gastric tissue by HE staining were observed under light microscope, and immunohistochemistry was used to detect the expressions of MEK1/2 and ERK1/2 protein in gastric tissue of rats. Results Compared with the blank control group rats, the gastric mucosa injury in the model group was obvious, which showed that the damage and abscission was more serious; compared with the model group, the gastric mucosa of rats was partly exfoliated and the damage was improved in three zhuang group, and the surface of gastric mucosa of rats was more complete and damage was improved obviously in six zhuang group and nine zhuang group; compared with the blank control group, the expressions of MEK1/2 and ERK1/2 protein in gastric tissue increased obviously in other groups (P<0.01);compared with three zhuang group, the expressions of MEK1/2 and ERK1/2 protein in gastric tissue increased in six zhuang group and nine zhuang group (P<0.01), but the effects of the two group were similar, without statistical significance (P>0.05). Conclusion Ginger-separated moxibustion can repair gastric mucosa in rats with spleen deficiency, which may be closely associated with its effect in increasing the expressions of MEK1/2 and ERK1/2 protein in gastric tissue and activating the MEK/ERK signal transduction pathway.
