Effect of water extract from Jiangtang Decoction on PI3K/Akt signal pathway of skeletal muscle metabolism in KK-Ay diabetic mice
10.7501/j.issn.1674-6376.2017.10.006
- VernacularTitle:中药降糖复方水提取物对KK-Ay糖尿病小鼠骨骼肌糖代谢PI3K/Akt信号通路的影响
- Author:
ni Jin HONG
1
;
wei Wei LI
;
Ning ZHANG
;
Hong FU
;
mei Xue WANG
Author Information
1. 北京大学第一医院中西医结合研究室
- Keywords:
Jiangtang Decoction;
Type 2 Diabetes;
carbohydrate metabolism;
skeletal muscle;
PI3K/Akt;
glycogen synthase kinase -3β (GSK-3β);
glucose transporter-4 (GLUT-4);
glycogen synthase (GS);
insulin receptor substrate (IRS-1)
- From:
Drug Evaluation Research
2017;40(10):1397-1401
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of water extract from Jiangtang Decoction (WEJTD) on PI3K/Akt signal pathway of skeletal muscle metabolism in KK-Ay diabetic mice.Methods Totally 50 KK-Ay mice were randomly divided into five groups:model group,metformin (positive drug,250 mg/kg) group,WEJTD low,medium,and high dose (2,4,and 8 g/kg) group,with 10 C57BL/6J mice as normal group.The relative drugs were ig administered once a day for 12 weeks,and mice in control group and model group were perfused with distilled water of equal volume.After 12 weeks' oral administration,mice were executed to separate serum,serum insulin level was detected by ELISA kit method;RNA was extracted from muscle tissue by Trizol,and real-time PCR were used to detect the level of PI3K,Akt,GLUT-4,GSK-3β,GS and IRS-1 mRNA.Results WEJTD can down-regulate concentration of insulin in serum and GSK-3β mRNA in skeletal muscle (P ＜ 0.05 and 0.001),and down-regulate mRNA of PI3K,Akt,IRS-1,GLUT-4,and GS in skeletal muscle (P ＜ 0.05,0.01,and 0.001).Conclusion WEJTD decreased glycogen deposition and stimulated glucose transport in skeletal muscle through upregulation of PI3K/Akt signaling pathway.
