Effects of Ligustrazine on proliferation of human retinal vascular endothelial cells induced by high glucose
10.7501/j.issn.1674-6376.2017.10.009
- VernacularTitle:川芎嗪对高糖诱导的人视网膜血管内皮细胞增殖的影响
- Author:
Ni YAN
1
;
long Zhen LI
Author Information
1. 鄂东医疗集团黄石市中心医院(湖北理工学院附属医院)眼科
- Keywords:
Ligustrazine;
retinal vascular endothelial cells;
cell cycle;
VEGF;
cell proliferation
- From:
Drug Evaluation Research
2017;40(10):1414-1417
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Ligustrazine on proliferation of human retinal vascular endothelial cells (HRCECs) induced by high glucose.Methods HRCECs cells were divided into control (saline) group,model (25 mmol/L glucose) group,ligustrazine low,medium,high concentration (50,100 and 200 mol/L) group,cultured for 48 h.Cell proliferation was detected by MTT cytotoxicity assay.Flow cytometry was used to detect cell cycle,and the expression of VEGF was detected by ELISA analysis.Results Compared with control group,HRCECs cell proliferation,cell division (M) phase ratio and supematant of VEGF concentration in model group increased significantly (P ＜ 0.05,0.01).Compared with model group,cell proliferation,M phase ratio and supematant of VEGF in Ligustrazine low,medium and high concentration groups decreased significantly (P ＜ 0.05,0.01),and showed a concentration dependence.Conclusion Ligustrazine can inhibit the expression of VEGF in HRCECs cells induced by high glucose to block cell cycle and inhibit the proliferation of HRCECs cells.
