In Silico Cloning and Bioinformatics Analysis of Chalcone Synthase Gene from Ribes Americanum
- VernacularTitle:美洲茶藨子查耳酮合成酶基因电子克隆和生物信息学分析
- Author:
Weichao REN
1
,
2
;
黑龙江中医药大学药学院
;
Dong WEN
;
Rui ZHAO
;
Wei MA
Author Information
1. 伊春林业科学院 黑龙江伊春153000
2. 黑龙江中医药大学药学院
- Keywords:
Ribes americanum;
Chalcone synthase;
In silico cloning;
Bioinformatics
- From:
China Pharmacist
2017;20(12):2106-2109
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the in silico cloning and bioinformatics of chalcone synthase gene from Ribes americanum. Meth-ods:Ribes nigrum chalcone synthase sequence and Ribes americanum were cloned by retrieving the EST database as the querying probe, which were assembled by CAP3 sequence assembly program, and the bioinformatics database and related software were used to predict the structure and perform the function analysis. Results:Bioinformatical analysis showed chalcone synthase gene encoded 1423 bp and contained a 1173bp ORF, the protein was with 390 amino acid, which was a hydrophilic protein located in cytoplasm including three transmembrane regions with the secondary structure composed of alpha helix. Conclusion:The study is helpful to the further ex-planation for the molecular function mechanism of chalcone synthase gene from Ribes americanum.
