Optimization of Ultrasonic Extraction Process of Total Alkaloids from Oxytropis Falcata Bunge by Central Composite Design and Response Surface Method
- VernacularTitle:星点设计-效应面法优化镰形棘豆总生物碱的超声提取工艺
- Author:
Qiping ZENG
1
;
Yixiang CAO
;
Jie LI
;
Zhixiong LAN
;
Jinshan CHEN
Author Information
1. 解放军第175医院/厦门大学附属东南医院 福建 漳州363000
- Keywords:
Oxytropis falcate Bunge;
Total alkaloids;
Acid dye colorimetry;
Central composite design and response surface method;
Extraction technology
- From:
China Pharmacist
2017;20(12):2122-2126
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the ultrasonic extraction process of total alkaloids from Oxytropis falcata bunge. Methods:The independent variables were solvents ratio, extracting time and ethanol concentration, and the dependent variable was content of total al-kaloids. Based on single factor tests, central composite design and response surface methodology was adopted to optimize the extraction technology. Results:The optimal extraction conditions were as follows: extracted twice with 36-fold amount of 72% ethanol ( contai-ning 1% acetic acid) at 60 ℃, and extracted 77 minutes each time. Under the above conditions, the content of total alkaloids was 2. 793 mg·g-1 with the bias ratio less than 2% when compared with the model predictions. Conclusion:Ultrasonic extraction process of total alkaloids from Oxytropis falcata Bunge optimized by central composite design and response surface method is simple, highly pre-cise, reliable and predictable.
