Effects of oat β-glucan on the expression of small intestine injury-related genes in septic rats
10.3969.j.issn.1671-7856.2017.12.007
- VernacularTitle:燕麦β-葡聚糖对脓毒症小肠损伤相关基因表达的影响
- Author:
peng Kun WU
1
;
Ying CHEN
;
jia Zhi HUANG
;
hong Cai YAN
Author Information
1. 南华大学附属第二医院
- Keywords:
Oat β-glucan;
Sepsis;
Intestinal mucosa
- From:
Chinese Journal of Comparative Medicine
2017;27(12):39-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of oatβ-glucan on gene expression in small intestine injury in sepsis. Methods SD rats were randomly divided into normal control group ( NC group) , experimental control group ( TC group) and oat β-glucan group ( Oglu group) . TC group and Oglu group were used to establish sepsis model with CLP method ,and oglu group with different doses of oatβ-glucan. After 12 h the small intestine tissue were collected. Western blot was used to detect gene expression in the small intestine tissue injury. Results 1 ) Oat β-glucan reduced sepsis-induced small intestine injury,and reduced the TNF-α, IL-1βand IL-6 levels in small intestine of septic rats. (2) The expression of small intestinal injury-related proteins such as Bax, caspase-3, Toll-like, apoptotic genes FAS and FASL and NF-κB were significantly increased,and Bcl-2 expression was down-regulated in the TC group. (3) The expressions of Bax, caspase-3, Toll-like, apoptotic genes FAS and FASL and NF-κB in oat β-glucan-treated rats were lower than that in the septic rats, while Bcl-2 expression was higher than the TC group. Conclusions Oat β-glucan regulates the expression of small intestinal injury genes in septic rats and protects the small intestinal epithelium against sepsis-induced injury.
