Screening and identification of mRNAs targeted by human cytomegalovirus(HCMV)-encoded miR-US33-1-5p in HCMV-infected human embryo lung fibroblast cells
10.3760/cma.j.issn.0254-5101.2017.10.009
- VernacularTitle:人巨细胞病毒miR-US33-1-5 p在感染的人胚肺成纤维细胞中靶mRNA的筛选与鉴定
- Author:
Xin GUO
1
,
2
;
110032 沈阳,中国医科大学附属第四医院儿科
;
Ying QI
;
Yujing HUANG
;
Zhongyang LIU
;
Yanping MA
;
Yaozhong SHAO
;
Shujuan JIANG
;
Qiang RUAN
Author Information
1. 110004 沈阳,中国医科大学附属盛京医院病毒研究室
2. 110032 沈阳,中国医科大学附属第四医院儿科
- Keywords:
Human cytomegalovirus;
miRNA;
Target mRNA
- From:
Chinese Journal of Microbiology and Immunology
2017;37(10):772-777
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and identify mRNAs targeted by human cytomegalovirus-encoded miR-US33-1-5p (HCMV miR-US33-1-5p) for understanding the biological functions of HCMV miR-US33-1-5p. Methods Potential target mRNAs of HCMV miR-US33-1-5p were screened out by using hybrid-PCR, a simple and effective method. Dual-Luciferase Reporter Assay System was used to identify the binding abili-ties of HCMV miR-US33-1-5p to 3′-UTRs of these potential target mRNAs. Results Twelve potential mRNAs targeted by HCMV miR-US33-1-5p were screened out in human embryo lung fibroblast cells infected with HCMV. Luciferase activities of 3′-UTRs of seven target mRNAs were significantly down-regulated. Conclusion Proteins encoded by these target mRNAs are involved in virus replication,immune system reg-ulation,protein synthesis,cell cycle regulation,energy metabolism and so on. Identification of mRNAs tar-geted by HCMV miR-US33-1-5p is helpful for further studies on biological functions of miR-US33-1-5p.
