Association of BCAS3 rs11653176 gene polymorphism with the occurrence of gout
10.3760/cma.j.issn.1000-6699.2017.11.007
- VernacularTitle:BCAS3基因rs11653176位点单核苷酸多态性与痛风的相关性研究
- Author:
Fengxiu NIU
1
;
Jianxia HU
;
Yangang WANG
;
Xiaoqi SONG
;
Huiwen ZHAO
;
Changgui LI
;
Zhaoxia LI
;
Shilu XU
;
Zhengju FU
Author Information
1. 266003,青岛大学附属医院内分泌科
- Keywords:
Gout;
BCSA3 gene;
Polymorphism;
single nucleotide;
Inflammatory factors
- From:
Chinese Journal of Endocrinology and Metabolism
2017;33(11):950-954
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the polymorphisms of the primary gout predisposing gene (BCAS3) rs11653176 locus and the incidence of gout in Han Chinese men in coastal areas of Shandong Province. Methods One hundred and fifty-two cases of patients with gout remission,68 cases of acute stage,252 patients with hyperuricemia, and 280 healthy subjects, total males, were enrolled. Genotyping the rs11653176 locus of BCAS3 gene by TaqMan probe technique. The expression level of BCAS3 gene mRNA in each PBMC was measured by RT-qPCR. The levels of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), and interleukin-18(IL-18)in serum were measured by ELISA. Results The change of allele frequency of rs11653176 locus in BCAS3 gene was associated with gout(P<0.01). BCAS3 mRNA in patients with gout was significantly higher than that of healthy people and patients with hyperuricemia(P<0.01). In gout patients, the expression level of BCAS3 gene containing C allele was higher than that of T allele(mRNA,P<0.05). The inflammatory factors in the acute phase of gout were significantly higher than those in phases of remission and hyperuricemia(P<0. 01). Conclusion Changes in the allele frequency of BCAS3 alleles rs11653176(high C, low T)may contribute to the expression of this gene,and lead to gout. And the onset of gout is closely related to the production of inflammatory factors.
