Effects of growth differentiation factor 11 on angiogenesis in diabetic hindlimb ischemia
10.3760/cma.j.issn.1000-6699.2017.11.011
- VernacularTitle:生长分化因子11对糖尿病下肢缺血大鼠血管新生的作用研究
- Author:
Jiajia ZHANG
1
;
Guangda XIANG
;
Huan LI
;
Biao ZHU
;
Min LIU
;
Lin XIANG
;
Jing DONG
Author Information
1. 南方医科大学附属武汉临床学院
- Keywords:
Growth differentiation factor 11;
Diabetes mellitus;
experimental;
Hindlimb ischemia
- From:
Chinese Journal of Endocrinology and Metabolism
2017;33(11):965-969
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and possible mechanism of growth differentiation factor 11 (GDF11)on angiogenesis in diabetic hindlimb ischemia. Methods Sixty SD rats were used in this study. Diabetes was induced by intraperitoneal injection of streptozotocin. 3 days after streptozotocin administration, 40 rats with plasma glucose concentration≥16.7 mmol/L were selected in the subsequent experiments. 12 weeks after diabetes was induced,the left femoral artery and all the sides branches were dissected free and excised. After resection of the left femoral artery,rats were randomized to four groups:PBS group(n=10),GDF11 group(n=10),IgG Ab group (n=10),or GDF11 Ab group(n=10). After 0,7,and 14 days,the serial blood flows were measured by a Laser Doppler perfusion image(LDPI)analyzer. To detect capillary endothelial cell,the sections of muscles were reacted with anti-CD31 monoclonal antibodies,and subsequently reacted with Cy3-conjugated anti-rabbit IgG antibody. The expression levels of HIF1α and VEGF were detected by western blotting. Results In GDF11 group significantly increased the blood perfusion and capillary density of ischemia hindlimb of the diabetic rats were found,which was correlated to an increased level of HIF1α and VEGF. In contrast, GDF11 Ab could lead to the opposite effects. Conclusion GDF11 treatment promotes the recovery of diabetic hindlimb ischemia, which may be related to the improvement of expression of HIF1 alpha and VEGF.
