Study of PAK4 induces epithelial-mesenchymal transition of breast cancer cells
10.3969/j.issn.1000-484X.2017.12.003
- VernacularTitle:p21蛋白激活激酶4在乳腺癌上皮间质转化中的作用研究
- Author:
Guang Xu MI
1
;
Qing Shou LI
;
Lei LIU
;
Dan Xiao LU
;
Qiu Yan FANG
;
Yan TAN
Author Information
1. 吉林省人民医院医学诊治实验中心
- Keywords:
Breast cancer;
p21 protein activated kinase 4;
Epithelial-mesenchymal transition
- From:
Chinese Journal of Immunology
2017;33(12):1771-1773,1778
- CountryChina
- Language:Chinese
-
Abstract:
[Abstartc] Objetcive: To analyze the effect of p21 protein activated kinase 4( PAK4) on epithelial-mesenchymal transition of breast cancer cells.Met hods: The mRNA and protein expression of PAK4,E-Cadherin,N-Cadherin and Vimentin were detected by qRTP-CR and Western blot in breast cancer cells transfected with pcDNA -PAK4 or siRNA PAK4;The biology behaviors of MCF-7 cells and MDA-MB-231 cells transfected with pcDNA-PAK4 or siRNA PAK4 were analysed by cell migration assay ,invasion assay.Results:Overexpressing PAK4 could significant increase in the migration and invasion compared with vector-infected cells, decrease the expression of epithelial marker E-cadherin and upregulate the expression of mesenchymal markers N-cadherin and Vimentin in detached MCF7 cells.Silenced PAK4 gene in detached MDA-MB-231 cells could suppress the migration and invasion ,decrease the levels of the mesenchymal markers and increased the levels of the epithelial markers at both mRNA and protein levels .Conclusion:PAK4 plays a key role in EMT of breast cancer cells ,and its promoting EMT effect associated with upregulating the expression of Slug .
