Cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) enhances humoral immune responses against norovirus(GⅡ.4) virus-like particles
10.3760/cma.j.issn.0254-5101.2017.10.007
- VernacularTitle:cGAMP可显著增强针对诺如病毒(GⅡ.4)病毒样颗粒抗原的体液免疫应答
- Author:
Jing CHEN
1
;
Meiying LIU
;
Chongfa TANG
;
Yu LIU
;
Youxiu ZHONG
;
Fang TANG
;
Zhijing MA
;
Junwei HOU
;
Bo WEI
Author Information
1. 101111,北京生物制品研究所有限责任公司第五研究室
- Keywords:
cGAMP;
Norovirus;
Virus like particles (VLPs);
Aluminum adjuvant
- From:
Chinese Journal of Microbiology and Immunology
2017;37(10):761-765
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the immunopotentiating effect of cyclic guanosine monophos-phate-adenosine monophosphate (cGAMP) as an adjuvant on norovirus (GⅡ. 4) virus like particles (VLPs) in the development of norovirus vaccine. Methods BALB/c mice were intramuscularly immunized with norovirus (GⅡ.4) VLPs composed of capsid protein VP1 in combination with cGAMP or Al(OH)3. Norovirus VLPs-specific antibodies in serum were detected by ELISA. A synthetic histo-blood group antigen (HBGA)-VLPs blocking assay was used to analyze neutralizing antibodies against norovirus VLPs in serum samples. Results Immunization with norovirus VLPs in the presence of cGAMP induced a strong humoral immune response in BALB/c mice. Levels of specific IgG antibodies in serum induced by using cGAMP as the adjuvant were significantly higher than those induced by using Al(OH)3adjuvant when immunization of BALB/c mice with the same dosage of VLPs. The antibody level induced by 1 μg of VLPs in combination with cGAMP was equivalent to that elicited by 10 μg of VLPs combined with Al(OH)3adjuvant. Results of the synthetic HBGA-VLPs blocking assay showed that the blocking rate in cGAMP+VLPs immunization group were significantly higher than that in Al(OH)3+VLPs immunization group when using the same dosage of VLPs. No significant difference in blocking rate was observed between cGAMP+VLPs(1 μg) and Al(OH)3+VLPs (10 μg) immunization groups. Conclusion cGAMP significantly enhanced the specific humoral immune response induced by norovirus (GⅡ.4) VLPs in mice as compared with Al(OH)3adjuvant. It might be used as a novel adjuvant to replace the traditional aluminum adjuvant in the development of norovir-us vaccine.
