Research of inhibitory mechanism of salidroside B on proliferation and metastasis of gastric cancer SNU-1 cells
10.3969/j.issn.1000-484X.2017.12.012
- VernacularTitle:五味子乙素抑制胃癌SNU-1细胞增殖和转移的作用机制研究
- Author:
Ling Yue LI
1
;
Feng Gao WANG
;
Hong PENG
;
Chuan Hui LI
Author Information
1. 黄河科技学院医学院
- Keywords:
salidroside B;
Gastric cancer SNU-1 cells;
Inhibitory effect;
ROS;
Caspase-3;
MMP-9
- From:
Chinese Journal of Immunology
2017;33(12):1815-1818,1827
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the related mechanism of salidroside B on proliferation and metastasis of Rhodiola glucoside glioma cell.Methods:The inhibitory rate of salidroside B on SNU-1 cells were determined using MTT method .The effect of salidroside B on inhibiting metastasis of SNU-1 cells was determined by transwell assay .Finally the effect of expression of related proteins of SNU-1 cells was discussed.The SNU-1 cells apoptosis rate was detected by flow cytometry .Results: The growth inhibitory rates of SNU-1 cells were increased with the increasing of dose of salidroside B (10,50,100 μg/ml),and there were significant differ-enced.Apoptosis flow cytometry test results showed that SNU-1 cells apoptosis rate was increased with the increasing of dose of salidroside B(10,50,100 μg/ml).ROS levels was increased with the increase of the concentration of salidroside with significant differ -ence.The expression of salidroside B on Caspase-3,Bax and E-cadherin of SNU-1 cells were increased,Bcl-2,N-cadherin and MMP-9 of SNU-1 cells were decreased.Conclusion:The apoptosis of SNU-1 cells may be through Caspase-3 pathway,mitochondrial pathway apoptosis cascade and the level of ROS by salidroside B , and the reduce of metastasis ability may also through the destruction of adhesion between the cells and basement membrane and increased the stability of tumor cells by salidroside B .
