Forensic Investigation in Han Population by SiFaTM 23 Plex Kit (Beta Version)
10.3969/j.issn.1004-5619.2017.05.015
- VernacularTitle:SiFaTM23Plex试剂盒(提取测试版)在汉族人群中的法医学调查
- Author:
hu Wu GONG
1
;
hua Shao XUE
;
Yan ZHANG
;
xin Ru ZHU
;
min Zhen ZHAO
Author Information
1. 西安市公安局刑事侦查局技术处
- Keywords:
forensic genetics;
short tandem repeat;
SiFaTM 23 Plex Kit (beta version);
Han nationality
- From:
Journal of Forensic Medicine
2017;33(5):516-521
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the performance of SiFaTM 23 Plex Kit (beta version) and its population genetics of STR loci in Han population.Methods Genotyping was performed for 1 000 unrelated healthy Han individuals by the kit.The efficiency of the kit was tested,and the frequency data and population genetics parameter information of STR loci were analysed statistically.Results The minimum amplification system could be 6.25 μL.In 25 μL standard reaction system,a satisfied genotyping profiles could be obtained with the DNA content as low as 125 pg.Among the 1 000 individuals,267 alleles were detected by 21 autosomal STR loci of the kit,which conformed to Hardy-Weinberg equilibrium.Fifteen and eleven alleles were observed at the newly added STR loci D1S1656 and D10S1248,respectively,which showed a high polymorphism information content.Conclusion SiFaTM 23 Plex Kit (beta version) is excellent in testing blood samples.Its accuracy,repeatability and sensitivity can satisfy the need of forensic practice,which makes it be applied to forensic-related case work and DNA database establishment.
