Application of RT-qPCR in the Study of Forensic Pathology

VernacularTitle:RT-qPCR技术在法医病理学研究中的应用
Author: hao Si DU ¹ ; ri Dong LI ; jun Hui WANG ; Qi WANG
Author Information

1. 南方医科大学法医学院
Keywords: forensic pathology; reverse transcriptase polymerase chain reaction; review; real-time quantification
From: Journal of Forensic Medicine 2017;33(5):526-531
CountryChina
Language:Chinese
Abstract: Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a convenient and highly efficient method for the detection of mRNA in tissues or body fluid samples.It has the characteristics of easy operation,high sensitivity and specificity,etc.With a wide application in medicine,biology and other fields,RT-qPCR technique has made some progresses in the research field of forensic pathology.This paper reviews the application value of RT-qPCR in the study of forensic pathology and current situation,as well as the research progress at home and abroad reviews.It also summarizes the notes of samples extraction,RT-qPCR experiments and data processing,which aims to provide reference for the forensic research and its application.