Therapeutic evaluation of systemic photodynamic therapy in a rat model for acute myeloid leukemia
10.3760/cma.j.issn.1673-4181.2017.05.003
- VernacularTitle:系统性光动力疗法对急性髓细胞性白血病大鼠的疗效评价
- Author:
Xiafei SHI
1
;
Wendong JIN
;
Hongjun WU
;
Huijuan YIN
;
Huajiang DONG
;
Qiang WANG
Author Information
1. 中国医学科学院北京协和医学院生物医学工程研究所
- Keywords:
Photodynamic therapy;
Leukemia;
Green fluorescent protein;
Cytokines;
Extracorporeal circulation
- From:
International Journal of Biomedical Engineering
2017;40(5):323-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the efficacy of the systemic photodynamic therapy (SPDT)for treating leukemia using a Brown Norway myeloid leukemia (BNML) rat model.Methods The BNML rat model was established by injecting green fluorescent protein (GFP)-LT12 cells into the tail vein.After GFP-LT12 injection,the early-SPDT group,mid-SPDT group and late-SPDT group were treated with SPDT at 5,10 and 15 days,the negative control group was fed as usually,and the Ara-c positive control group was treated with Ara-c at 7 days.The GFP-LT12 cells were traced by a fluorescence imaging system.The GFP-LT12 cells in the tissues and organs were detected by flow cytometry.The levels of IFN-γ,IL-1α,IL-1β,IL-2,IL-4,IL-6,IL-10 and TNF-α in serum were detected by milliplex rat cytokine 9 kits.Results Compared with the negative control group,the survival times of the rats in the earlySPDT group,mid-SPDT group and the late-SPDT group were prolonged (all P＜0.05).The ratios of GFP-LT12 cells in pulp and liver were decreased in the late-SPDT group.The levels of IL-1β,IL-10,TNF-α and IFN-γin serum of the late-SPDT group were decreased (all P＜0.05).Conclusion The SPDT is an effective method for the treatment of leukemia,and the anti-tumor immune effect may play a key role in this process.
