Effects of ropivacaine on proliferation,apoptosis and cell cycle of MDA-MB-231 cells
10.3969/j.issn.1006-5725.2017.21.007
- VernacularTitle:罗哌卡因对乳腺癌MDA-MB-231细胞生长、凋亡诱导和周期阻滞的影响
- Author:
Ningning JI
1
;
Ming XIA
;
Jingyu HUA
;
Lei DU
;
Yongmei ZHANG
Author Information
1. 221004,徐州医科大学江苏省麻醉学重点实验室
- Keywords:
popivacaine;
MDA-MB-231 cells;
proliferation;
apoptosis;
cell cycle
- From:
The Journal of Practical Medicine
2017;33(21):3527-3530
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ropivacaine on proliferation,apoptosis and cell cycle of MDA-MB-231 Cells. Methods The cultured MDA-MB-231 cells were treated with different concentrations of ropivacaine. The proliferation of MDA-MB-231 cells was detected by MTT method. Cell apoptosis and cell cycle were detected by Annexin V-FITC/PI and flow cytometry. Results After ropivacaine administration,MDA-MB-231 cells proliferation inhibition ratio increased significantly.Annexin V-FITC/PI and flow cytometry showed ropiva-caine had no significant effects on cell apoptosis and cell cycle.Conclusion Ropivacaine can inhibit the prolifera-tion of MDA-MB-231 cells,but can′t induce apoptosis and block cell cycle.
