Effects of miR-143 on invasion and migration of B cell lymphoma cells through regulating expression of PAN3 protein
10.3969/j.issn.1000-484X.2017.12.004
- VernacularTitle:miR-143调控PAN3蛋白的表达对B细胞淋巴瘤细胞侵袭和迁移的影响
- Author:
Man YANG
1
;
Sun WU
;
Xiu Ling ZHANG
;
Yan HUANG
;
Mei You ZI
;
Yuan ZHANG
Author Information
1. 新乡医学院第一附属医院血液科
- Keywords:
miR-143;
Lymphoma;
PAN3;
Transwell
- From:
Chinese Journal of Immunology
2017;33(12):1774-1778
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of miR-143 on the invasion and migration of B cell lymphoma cells .Methods:The expression of miR-143 in normal bone marrow and lymphoma was detected by qPCR .The expression levels of miR-143 in different cell lines were examined by qPCR .qPCR was used to detect the ability of miR-143 on PAN3.The relationship between miR-143 and PAN3 was detected by double luciferase assay .The effect of miR-143 expression on the migration ability of B cell lymphoma E 6-1 cells was examined by scratch test .The effect of miR-143 expression on the invasion ability of B cell lymphoma E 6-1 cells was exa mined by transwell test.Results:Compared with normal bone marrow ,miR-143 was down-regulated in B-cell lymphoma.Double luciferase assay showed that miR-143 could regulate the expression of PAN 3.Overexpression of miR-143 ,E6-1 cells significantly reduced the ability to attack and migrate.Conclusion:miR-143 can regulate the migration and invasion of B cell lymphoma cells by regulating the expression of PAN3.
