Effects of site specific antibody of Na+-Ca2+exchanger on calcium transient in single ventricular myocytes of rats
10.3969/j.issn.1000-484X.2017.12.008
- VernacularTitle:抗钠钙交换体特异位点抗体对大鼠单个心室肌细胞钙瞬变的影响
- Author:
Jie Xiao BAI
1
;
Wei Bo WU
Author Information
1. 山西医科大学生理学系
- Keywords:
Na+-Ca2+exchanger;
Antibody;
Calcium transient;
Ventricular myocyte;
L-type Ca2+channel
- From:
Chinese Journal of Immunology
2017;33(12):1795-1798
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of site specific (124 HNFTAGDLGP STIVGSAAFNMF145 ) antibody of Sodium calcium exchanger ( NCX) on calcium transient in single ventricular myocytes of normal adult rats .Methods: Isolated adult rat hearts were perfused using Langendorff method and single ventricular myocytes were then obtained .The ventricular myocytes were incubated with Fuar-2/AM and 2% bovine serum albumin for about 40 min and then,the fluorescence images were recorded when excitation wavelengths were 340 nm and 380 nm using ion imaging system.Fluorescence value F340/F380,length of cell shortening ,time to 90%restore( TR90 ) and calcium sensitivity ( ratio of F340/F380 and cell shortening ) were calculated.Results:The site specific antibody of NCX increased F340/F380 and decreased TR90 in single ventricular myocytes ,but had no more significant effect on calcium sensitivi-ty.Pretreatment with KB-R7943 or Nicardipine could significantly inhibit the TR 90 decrease or F340/F380 increase induced by the anti-body.Pretreating ventricular myocytes with combination of KB-R7943 and Nicardipine ,the antibody had no more significant effects on calcium transient.Conclusion:Site specific ( 124 HNFTAGDLGPSTIVGSAAFNMF145 ) antibody of NCX could increase calcium transient and accelerate the decrease of intracellular calcium during diastole ,which mainly related to its effects of activating L-type Ca2+channel and NCX.
