Inhibitory role of BANK in progression of experimental autoimmune encephalo-myelitis
10.3969/j.issn.1000-484X.2017.12.020
- VernacularTitle:B细胞衔接蛋白BANK在小鼠实验性自身免疫性脑脊髓炎中的作用
- Author:
Hua Gui JIN
1
;
Hua Hong LI
;
Xue LI
;
Manabu FUJIMOTO
;
Gao Qing ZHANG
Author Information
1. 延边大学医学院免疫学与病原生物学教研室
- Keywords:
B cell;
B cell adoptor protein;
BANK;
EAE
- From:
Chinese Journal of Immunology
2017;33(12):1854-1857,1861
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of B cell adoptor protein with ankyrin repeats ( BANK ) in experimental autoimmune encephalomyelitis ( EAE) .Methods: C57BL/6 mice and BANK-deficient ( BANK-/-) mice were immunized with MOG peptide in CFA,and then observed the clinical symptoms and pathological severity .Results: The percentages of CD4+T cells,CD8+T cells and regulatory T cells in brain and spleen were analyzed by flow cytometry .BANK-/-mice showed significantly higher score at the peak and the plateau phase compared with wild-type mice(P<0.05).HE staining showed more widespread areas of inflammation and demyelination in BANK-/-mice when compared to wild-type mice on day 16.In addition,the frequency of CNS-infiltrating CD8+T cells was markedly higher in BANK-/-mice than in wild-type mice.In addition,the percentage of CD8+T cells from spleen in BANK-/-mice was also increased compared with wild-type mice (P<0.05).By contrast,the percentage of regulatory T cells and the ratio of CD 4/CD8 T cells from spleen in BANK-/-mice were significantly lower than in wild-type mice(P<0.05).Conclusion:Thus,BANK expression in B cells can inhibit the development of EAE .
