Role of negative regulatory factor IRAKM in immune dysregulation of systemic lupus erythematosus
10.3969/j.issn.1000-484X.2017.12.021
- VernacularTitle:负性调控因子IRAKM在系统性红斑狼疮免疫调节异常中的作用
- Author:
Ge Yan WU
1
;
Jun Jin QIU
;
Xia Hong LIU
;
Bo ZHANG
;
Feng Yu GONG
Author Information
1. 深圳市坪山区人民医院病理科
- Keywords:
Systemic lupus erythematosus;
Interleukin-1 receptor associated kinase-M;
Immune regulation
- From:
Chinese Journal of Immunology
2017;33(12):1858-1861
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of interleukin-1 receptor associated kinase-M(IRAKM)in systemic lupus erythematosus ( SLE) and its relationship with immunoregulation .Methods:103 patients with SLE were divided into active stage group (n=55) and stable stage group(n=48) according to their disease activity score (SLEDAI),and 40 healthy persons were chosen as control group.Real-time quantitative PCR ( RT-PCR ) was used to detect the expression of IRAKM mRNA in peripheral blood monouclear cells of the three groups .The levels of anti ds-DNA antibody and anti Sm antibody in serum were detected by Enzyme linked immunosorbent assay(ELISA).The levels of serum complement C3 and C4 were measured by immune scattering turbidimetry .Factor analysis of variance was performed to analyze the differences of all the observed indicators in the three groups .The correlation between IRAKM and autoantibodies and complements of SLE was analyzed by Pearson or Spearman .Results: The expression level of IRAKM mRNA of SLE patients in the active stage group and stable stage group were significantly lower than that in the control group ( P<0.05).Moreover,the expression level of IRAKM mRNA in the active stage group was lower than that in the stable stage group ,and the difference was statistically significant ( P<0.05 ) .Compared with the control group , the levels of anti ds-DNA antibody and anti Sm antibody in the active stage group and stable stage group were significantly increased ( P<0.05 ) ,and the levels of complement C 3 and C4 were obviously lower(P<0.05).The changes of serum levels of autoantibodies and complements in the active stage group were more remarkable than those in the stable stage group ( P<0.05 ) .The expression level of IRAKM mRNA in the SLE patients was negatively correlated with the levels of anti ds-DNA antibody and anti Sm antibody (P<0.05).However,it was positively correlated with the levels of complement C3 and C4 ( P<0.05 ) .Conclusion: IRAKM participates in the immune regulation process of SLE through negative regulation,and its expression level is closely related to the degree of SLE disease activity .
