PrepFiler Express BTATM Lysis Buffer Combined with Silicon Microbeads for Rapid DNA Extraction from Bone

VernacularTitle:PrepFiler Express BTATM裂解液联合硅珠法快速提取骨骼DNA
Author: cheng Shao DING ¹ ; cai Huai ZHANG ; lin Lin GAO
Author Information

1. 杭州市公安局刑事科学技术研究所
Keywords: forensic genetics; bone; DNA; PrepFiler Express BTATM; silica-extraction method
From: Journal of Forensic Medicine 2017;33(5):514-515,521
CountryChina
Language:Chinese
Abstract: Objective To establish a convenient and rapid method for extracting DNA from bone.Methods Fifteen long bone samples were washed and sterilized.The skeletal fragments were obtained by electric drill,and lysed by PrepFiler Express BTATM lysis buffer.DNA was then manually extracted by silicon microbeads for further analysis.Results STR genotyping was successfully obtained in 14 out of the 15 samples,and the detection rate was 93.33％.Conclusion The method for DNA extraction from bone established in present study is convenient,quick,effective,and with a strong applicability,which is worth spreading and applying.