Lithium chloride promotes neuronal differentiation of rat bone marrow mesenchymal stem cells by modulating autophagy
10.3969/j.issn.1000-4718.2017.12.003
- VernacularTitle:氯化锂通过调节自噬通路促进大鼠骨髓间充质 干细胞向神经细胞分化
- Author:
yu Guang ZHANG
1
;
jie Yan JIA
;
Jun WANG
;
song San LI
;
hao Guo TANG
;
mei Ming WANG
;
wen Yi WANG
;
na Deng ZHU
Author Information
1. 郑州大学 第三附属医院儿童康复科
- Keywords:
Lithium chloride;
Mesenchymal stem cells;
Autophagy;
Neuron;
Differentiation
- From:
Chinese Journal of Pathophysiology
2017;33(12):2128-2133
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the influence of lithium chloride (LiCl) on the neuronal differentiation of rat bone marrow mesenchymal stem cells (MSCs), and to explore whether autophagy was involved in this process .METHODS:MSCs were isolated and cultured in vitro.The cells were divided into LiCl group and control group .MSCs were treated withβ-mercaptoethanol as an inducer for triggering the cells to differentiate into neurons .The expression of neuronal markers-neuron specific enolase (NSE) and microtubule-associated protein-2 (MAP-2), and autophagic marker-microtubule-associ-ated protein 1 light chain 3 ( LC3) were measured by immunofluorescence method and Western blot .An autophagy activator rapamycin and autophagy inhibitor 3-methyladenine (3-MA) were applied to modulate the autophagy in the LiCl treated-cells.The protein expression of NSE and MAP-2 were determined by Western blot .RESULTS: After induction, the ex-pression of NSE and MAP-2 were increased .The percentage of NSE-and MAP-2-positive cells and the expression of NSE and MAP-2 in the LiCl group were greater than those in control group (P<0.05).After induction, the number of LC3-positive dots and the expression of LC3-Ⅱin LiCl group were greater than those in control group (P<0.05).The expres-sion of NSE and MAP-2 increased when the autophagy was modulated by rapamycin in LiCl treated -cells, and on the contra-ry, the expression of NSE and MAP-2 were inhibited as autophagy was modulated by 3-MA.CONCLUSION: Lithium chloride may promote the neuronal differentiation of rat bone marrow mesenchymal stem cells by modulating autophagy .
