Study on the Purification Technology of Total Saponins from Asparagus cochinchinensis with Macroreticu-lar Resin
10.6039/j.issn.1001-0408.2017.34.30
- VernacularTitle:天冬总皂苷的大孔树脂纯化工艺研究
- Author:
Liang LIU
1
;
Jiang DU
;
Hua FENG
;
Lina DING
;
Donglin CHEN
;
Yingxin ZHOU
Author Information
1. 遵义医药高等专科学校药学系
- Keywords:
Asparagus cochinchinensis;
Total saponins;
Macroreticular resin;
Purification technology
- From:
China Pharmacy
2017;28(34):4868-4871
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the purification technology of total saponins from Asparagus cochinchinensis with macro-reticular resin. METHODS:Using content of total saponins from A. cochinchinensis as index,single factor test was used to investi-gate the macroreticular resin model,sampling adsorption time,mass concentration of the column,adsorption capacity,volume frac-tion and the amount of elution solvent,elution rate,and optimize the purification technology. And verification test was conducted. RESULTS:HPD-300 macroreticular resin showed strong absorption and desorption property. The optimal purification technology was that sampling adsorption time was 60 min,mass concentration of sample liquid was 0.1 g/mL,adsorption capacity was 120 mL(15 BV),it was eluded with 60% ethanol solution with 3 BV and elution rate was 4 BV/h. In the verification test,the average desorption rate of total saponins was 68.30%(RSD=0.95%,n=3). CONCLUSIONS:Optimized purification technology is sta-ble,feasible,and can easily separate and purify the total saponins from A. cochinchinensis.
