The role of PD0332991 in clonogenicity of mantle cell lymphoma cells
10.3969/j.issn.1000-8179.2017.22.754
- VernacularTitle:PD0332991在套细胞淋巴瘤细胞集落形成中的作用
- Author:
WANG FANG
1
;
ZHANG XINWEI
Author Information
1. 天津医科大学肿瘤医院生物技术研究室
- Keywords:
mantle cell lymphoma;
cyclin-dependent kinases 6;
cell cycle arrest;
PD0332991;
colony formation
- From:
Chinese Journal of Clinical Oncology
2017;44(22):1115-1119
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the function and mechanism of cyclin-dependent kinase 4/6 (CDK4/6) inhibitor PD0332991 on clo-nogenicity of mantle cell lymphoma (MCL) cells. Methods:The effect of PD0332991 on MCL cell cycle distribution was assessed by flow cytometry;Western blot was used to test expression level of Rb protein and phosphorylated Rb protein in MCL after treatment with PD0332991;colony forming assay was performed to test the role of PD0332991 and mitoxantrone and their combination on colo-ny forming activity in MCL. Results:Flow cytometry revealed that PD0332991 can increase G0/G1 phase MCL cells and significantly de-crease S phase cells, leading to G0/G1 cell arrest. Western blot confirmed that PD0332991 exerted no effect on Rb protein expression but suppressed levels of phosphorylated Rb protein. Colony forming assay showed that PD0332991 significantly suppressed colony for-mation and enhanced the effect of mitoxantrone on colony forming activity in MCL. Conclusion:This study revealed that CDK4/6 inhib-itor PD0332991 induced G0/G1 cell arrest and increased the effect of mitoxantrone on MCL clonogenicity by suppressing levels of phosphorylated Rb protein.
