Aptamers from Cell-based Selection for Bioanalysis and Bioimaging
10.11895/j.issn.0253-3820.171337
- VernacularTitle:基于Cell-SELEX的核酸适配体在生化分析与生物成像中的应用
- Author:
Lin Yu DU
1
;
Ting Liu MO
;
Sha Ya YI
;
Ping Li QIU
;
Hong Wei TAN
Author Information
1. 分子科学与生物医学实验室
- Keywords:
Aptamer;
Cell-SELEX;
Bioanalysis;
Bioimaging;
Tumor cells;
Review
- From:
Chinese Journal of Analytical Chemistry
2017;45(12):1757-1765
- CountryChina
- Language:Chinese
-
Abstract:
Aptamers are single-stranded oligonucleotides ( DNA or RNA ) selected through a technology termed "Systematic evolution of ligands by exponential enrichment" ( SELEX ) . In addition to high affinity and high specificity for their target molecules, aptamers have some advantages such as low molecular weight, easy synthesis, high chemical stability, low immunogenicity, and convenient modification. Based on the Cell-SELEX technique, a panel of aptamers which can specifically recognize target cell lines has been generated. By targeting specific membrane proteins in their native state, these aptamers can identify subtle molecular differences among different cell lines, thus have attracted a broad interest in biomedical research. In this review, we summarized the development of aptamers and their use in detection, profiling and imaging of tumor cells. Also, their perspectives were discussed.
